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Article

Engineering of yeast Put4 permease and its application to lager yeast for efficient proline assimilation.

Bioscience, biotechnology, and biochemistry - 1 Jun 2005

Omura Fumihiko, Fujita Atsushi, Miyajima Keiji, Fukui Nobuyuki

Abstract excerpt

The Saccharomyces cerevisiae Put4 permease is significant for the transport of proline, alanine, and glycine. Put4p downregulation is counteracted by npi1 mutation that affects the cellular ubiquitination function. Here we describe mutant Put4 permeases, in which up to nine lysine residues in the cytoplasmic N-terminal domain have been replaced by arginine. The steady-state protein level of the mutant permease...

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PMID: 15973048
DOI: 10.1271/bbb.69.1162

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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