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Article

2nSILAC for Quantitative Proteomics of Prototrophic Baker's Yeast.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Dannenmaier Stefan, Oeljeklaus Silke, Warscheid Bettina

Abstract excerpt

Stable isotope labeling by amino acids in cell culture (SILAC) combined with high-resolution mass spectrometry is a quantitative strategy for the comparative analysis of (sub)proteomes. It is based on the metabolic incorporation of stable isotope-coded amino acids during growth of cells or organisms. Here, complete labeling of proteins with the amino acid(s) selected for incorporation needs to be guaranteed to...

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PMID: 33950496
DOI: 10.1007/978-1-0716-1024-4_18

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