Article
Development of a TaqMan assay for the six major genotypes of hepatitis C virus: comparison with commercial assays.
Journal of medical virology - 1 Jan 2008
Engle Ronald E, Russell Rodney S, Purcell Robert H, Bukh Jens
Abstract excerpt
A quantitative real-time PCR assay was developed that detects genomic RNA from reference strains representing the six major genotypes of hepatitis C virus (HCV) with equal sensitivity and accurately measured HCV RNA in JFH1 HCV-infected Huh7.5 cells. The method is indirectly calibrated to the first international (WHO 96/790) HCV standard preparation and has a linear dynamic range of 10(2.6)-10(6.5) IU/ml. In...
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