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Article

Development of hepatitis C virus genotyping by real-time PCR based on the NS5B region.

PloS one - 13 Apr 2010

Nakatani Sueli M, Santos Carlos A, Riediger Irina N, Krieger Marco A, Duarte Cesar A B, Lacerda Marco A, Biondo Alexander W, Carrilho Flair J, Carilho Flair J, Ono-Nita Suzane K

Abstract excerpt

BACKGROUND: Hepatitis C virus (HCV) genotyping is the most significant predictor of the response to antiviral therapy. The aim of this study was to develop and evaluate a novel real-time PCR method for HCV genotyping based on the NS5B region. METHODOLOGY/PRINCIPAL FINDINGS: Two triplex reaction sets were designed, one to detect genotypes 1a, 1b and 3a; and another to detect genotypes 2a, 2b, and 2c. This approach...

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Identifiers

PMID: 20405017
DOI: 10.1371/journal.pone.0010150
PMCID: PMC2854153

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Source receipt: pubmed, retrieved 7 Sept 2026.

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