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High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

1 Feb 1999

Abstract excerpt

We describe a rapid and reproducible method for assessment of the hepatitis C virus (HCV) load in serum samples. The method combines Taqman technology (Roche) and the ABI Prism 7700 (Perkin Elmer) real-time sequence detection system. We have optimized a single-tube reverse transcription-PCR (RT-PCR) that contains a dual-labeled fluorogenic probe to quantify the 5' noncoding region (5' NCR) of HCV. The probe...

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PMID: 9889212
DOI: 10.1128/jcm.37.2.327-332.1999

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