Article
Expression of the cloned gene for enterotoxin STb of Escherichia coli.
Infection and immunity - 1 Apr 1990
Lawrence R M, Huang P T, Glick J, Oppenheim J D, Maas W K
Abstract excerpt
This study involved the construction of hybrid plasmids to produce heat-stable enterotoxin type II of Escherichia coli (STb). The translation of the open reading frame for the STb gene estA was demonstrated in several ways. Studies using in vivo labeling with [35S]cysteine demonstrated a radiolab...
Topics
- Animals
- Bacterial Toxins
- Blotting, Northern
- Cloning, Molecular
- Enterotoxins
- Escherichia coli Proteins
- Gene Expression
- Hemocyanins
- Immune Sera
- Mutation
- Plasmids
- Rabbits
- Recombinant Fusion Proteins
- Swine