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Shiga toxin A subunit mutant of Escherichia coli O157:H7 releases outer membrane vesicles containing the B-pentameric complex.

FEMS immunology and medical microbiology - 1 Apr 2010

Kim Sang-Hyun, Lee Sang-Rae, Kim Keun-Su, Ko Ara, Kim Ekyune, Kim Yong-Hwan, Chang Kyu-Tae

Abstract excerpt

Shiga toxins (STx) are secreted extracellularly through the outer membrane vesicles (OMVs) of Escherichia coli O157:H7. In an attempt to produce STxA-deficient OMVs from E. coli O157:H7, site-specific deletions of the stx1A and stx2A subunit genes were carried out. The STxA-deficient phenotype of the stx1A/stx2A mutant was confirmed by Vero cell cytotoxicity and VTEC-RPLA assay. Western blot analyses showed that...

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PMID: 20199568
DOI: 10.1111/j.1574-695X.2010.00654.x

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