Article
Expression of the GTPase activating domain of the neurofibromatosis type 1 (NF1) gene in Escherichia coli and role of the conserved lysine residue.
The Journal of biological chemistry - 25 May 1992
Wiesmüller L, Wittinghofer A
Abstract excerpt
A small catalytic domain from the neurofibromatosis type 1 gene, NF1-333, consisting of 333 amino acids between residues 1197 and 1528, including an additional N-terminal methionine, was expressed in Escherichia coli as a soluble protein. Its catalytic activity under non-saturating conditions is similar to the full-length p120-GAP but different from truncated GAP-334. Under saturating conditions its kcat and KM...
Topics
- Amino Acid Sequence
- Base Sequence
- Catalysis
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Escherichia coli
- GTP Phosphohydrolases
- Genes, Bacterial
- Genes, Neurofibromatosis 1