Article
pK(a) calculations for class C beta-lactamases: the role of Tyr-150.
Proteins - 1 Jul 2000
Lamotte-Brasseur J, Dubus A, Wade R C
Abstract excerpt
The Poisson-Boltzmann method was used to compute the pK(a) values of titratable residues in a set of class C beta-lactamases. In these calculations, the pK(a) of the phenolic group of residue Tyr150 is the only one to stand out with an abnormally low value of 8.3, more than one pK(a) unit lower than the measured reference value for tyrosine in solution. Other important residues of the catalytic pocket, such as...
Topics
- Catalytic Domain
- Citrobacter freundii
- Enterobacter cloacae
- Escherichia coli
- Gammaproteobacteria
- Hydrogen Bonding
- Hydrogen-Ion Concentration
- Models, Molecular
- Mutation
- Static Electricity
- Tyrosine