Article
Nucleoside diphosphate kinase from Escherichia coli.
Journal of bacteriology - 1 May 1995
Almaula N, Lu Q, Delgado J, Belkin S, Inouye M
Abstract excerpt
Nucleoside diphosphate (NDP) kinase from Escherichia coli was purified to homogeneity and was crystallized. Gel filtration analysis of the purified enzyme indicated that it forms a tetramer. The enzyme was phosphorylated with [gamma-32P]ATP, and the pH stability profile of the phosphoenzyme indicated that two different amino acid residues were phosphorylated. Both a histidine residue and serine residues,...
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