Structural basis for nuclear import complex dissociation by RanGTP.

Abstract excerpt

Nuclear protein import is mediated mainly by the transport factor importin-beta that binds cytoplasmic cargo, most often via the importin-alpha adaptor, and then transports it through nuclear pore complexes. This active transport is driven by disassembly of the import complex by nuclear RanGTP. The switch I and II loops of Ran change conformation with nucleotide state, and regulate its interactions with nuclear...

Topics

• Active Transport, Cell Nucleus
• Allosteric Regulation
• Binding Sites
• Crystallography, X-Ray
• Models, Molecular
• Multiprotein Complexes
• Mutation
• Protein Conformation
• Saccharomyces cerevisiae
• Structure-Activity Relationship
• beta Karyopherins