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Article

Maximization of dextransucrase activity expressed in E. coli by mutation and its functional characterization.

Biotechnology letters - 1 Jan 2008

Nam Seung Hee, Ko Eun Ah, Jang Suk Sang, Kim Do Won, Kim Se Yong, Hwang Dae Sung, Kim Doman

Abstract excerpt

A novel dextransucrase gene, DSRN, was obtained by ultrasoft X-ray treatment of the DSRB742 gene. The DSRN gene was further mutated via site-directed mutagenesis producing four mutants: DSRN1 (F196S), DSRN2 (Y346N), DSRN3 (K395T) and DSRN4 (P980T). Dextransucrases derived from DSRB742 and its mutants were expressed in E. coli and affinity-purified using dextran to give 80% purity. They had specific activities of...

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PMID: 17673948
DOI: 10.1007/s10529-007-9498-z

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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