Article
Endothelial cell activation of the smooth muscle cell phosphoinositide 3-kinase/Akt pathway promotes differentiation.
Journal of vascular surgery - 1 Mar 2005
Brown David J, Rzucidlo Eva M, Merenick Bethany L, Wagner Robert J, Martin Kathleen A, Powell Richard J
Abstract excerpt
OBJECTIVE: Interactions between endothelial cells (ECs) and smooth muscle cells (SMCs) are fundamental in diverse cardiovascular processes such as arteriogenesis, atherosclerosis, and restenosis. We aimed to determine the intracellular signaling mechanisms by which ECs promote a differentiated SMC phenotype. METHODS: Bovine thoracic aorta ECs and SMCs were isolated and cultured. For co-culture studies, ECs were...
Topics
- Animals
- Aorta, Thoracic
- Cattle
- Cell Differentiation
- Cells, Cultured
- Coculture Techniques
- Endothelial Cells
- Muscle, Smooth, Vascular
- Myocytes, Smooth Muscle
- Phenotype
- Phosphatidylinositol 3-Kinases
- Protein Serine-Threonine Kinases
- Protein-Tyrosine Kinases