Article
Discrimination of respiratory dysfunction in yeast mutants by confocal microscopy, image, and flow cytometry.
Cytometry - 1 Jan 1996
Petit P, Glab N, Marie D, Kieffer H, Métézeau P
Abstract excerpt
Living yeast cells can be selectively stained with the lipophilic cationic cyanine dye DiOC6(3) in a mitochondrial membrane potential-dependent manner. Our study extends the use of flow cytometric analysis and sorting to DiOC6(3)-stained yeast cells. Experimental conditions were developed that prevented the toxic side effect of the probe and gave a quantitative correlation between fluorescence and mitochondrial...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
