Article
Isolation and characterization of carotenoid hyperproducing mutants of yeast by flow cytometry and cell sorting.
Bio/technology (Nature Publishing Company) - 1 Jan 1991
An G H, Bielich J, Auerbach R, Johnson E A
Abstract excerpt
The carotenoid pigment astaxanthin (3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) is an important component in feeds of aquacultural animals. It is produced as a secondary metabolite by the yeast Phaffia rhodozyma, and the isolation of rare mutants that produce increased quantities is limited by the lack of genetic selections. As a model system for enriching mutants increased in production of secondary...
Topics
- Carotenoids
- Cell Separation
- Flow Cytometry
- Fluorescence
- Microscopy, Fluorescence
- Mutation
- Xanthophylls
- Yeasts
- beta Carotene