Article
Isolation of yeast mutants defective for localization of vacuolar vital dyes.
Proceedings of the National Academy of Sciences of the United States of America - 29 Sept 1998
Zheng B, Wu J N, Schober W, Lewis D E, Vida T
Abstract excerpt
An application of flow cytometric sorting is used for isolation of Saccharomyces cerevisiae mutants that mislocalize vacuolar vital dyes. This screen is based on the ability of a lipophilic styryl compound, N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrie nyl )pyridinium dibromi...
Topics
- Biological Transport, Active
- Endocytosis
- Endosomes
- Energy Metabolism
- Flow Cytometry
- Fluorescent Dyes
- Genes, Fungal
- Genetic Complementation Test
- Intracellular Membranes
- Microscopy, Fluorescence
- Mutation
- Phenotype
- Phosphatidylcholines
- Phosphatidylethanolamines
- Pyridinium Compounds
- Quaternary Ammonium Compounds
- Saccharomyces cerevisiae
- Temperature