Article
Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli.
The Journal of biological chemistry - 15 May 1992
Belunis C J, Raetz C R
Abstract excerpt
The enzyme 3-deoxy-D-manno-octulosonic acid (Kdo) transferase is encoded by the kdtA gene of Escherichia coli and plays a key role in lipopolysaccharide biosynthesis. It transfers Kdo from CMP-Kdo to lipid A or its tetraacyldisaccharide-1,4'-bisphosphate precursor, lipid IVA. Using a strain that...
Topics
- Base Sequence
- Carbohydrate Sequence
- Endotoxins
- Escherichia coli
- Genes, Bacterial
- Genotype
- Kinetics
- Lipid A
- Lipopolysaccharides
- Models, Biological
- Molecular Sequence Data
- Molecular Weight
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Restriction Mapping
- Substrate Specificity
- Transferases