A gene coding for 3-deoxy-D-manno-octulosonic-acid transferase in Escherichia coli. Identification, mapping, cloning, and sequencing.

Article

A gene coding for 3-deoxy-D-manno-octulosonic-acid transferase in Escherichia coli. Identification, mapping, cloning, and sequencing.

The Journal of biological chemistry - 25 May 1991

Clementz T, Raetz C R

Abstract excerpt

An autoradiographic assay applicable to colonies immobilized on filter paper was developed for obtaining temperature-sensitive mutants of Escherichia coli defective in the transfer of 3-deoxy-D-manno-octulosonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lip...

Topics

Autoradiography
Base Sequence
Cell-Free System
Cloning, Molecular
Escherichia coli
Genes, Bacterial
Molecular Sequence Data
Mutation
Plasmids
Transferases

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