Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.

Abstract excerpt

We have cloned and sequenced a cluster of six open reading frames containing gene kdsA from Escherichia coli K-12. The gene encodes 3-deoxy-D-manno-octulosonate 8-phosphate synthetase (KDO-8-phosphate synthetase), which catalyzes formation of 3-deoxy-D-manno-octulosonic acid (KDO), an essential c...

Topics

• Aldehyde-Lyases
• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• Cytoplasm
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Lipopolysaccharides
• Molecular Sequence Data
• Mutation
• Nucleotidyltransferases
• Operon
• Promoter Regions, Genetic