Article
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.
Science (New York, N.Y.) - 25 Feb 2005
Roosild Tarmo P, Greenwald Jason, Vega Mark, Castronovo Samantha, Riek Roland, Choe Senyon
Abstract excerpt
Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we...
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