Article
Yarrowia lipolytica mutants devoid of pyruvate carboxylase activity show an unusual growth phenotype.
Eukaryotic cell - 1 Feb 2005
Flores Carmen-Lisset, Gancedo Carlos
Abstract excerpt
We have cloned and characterized the gene PYC1, encoding the unique pyruvate carboxylase in the dimorphic yeast Yarrowia lipolytica. The protein putatively encoded by the cDNA has a length of 1,192 amino acids and shows around 70% identity with pyruvate carboxylases from other organisms. The corresponding genomic DNA possesses an intron of 269 bp located 133 bp downstream of the starting ATG. In the branch motif...
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