Isolation of a yeast mutant deficient in pyruvate carboxylase activity.

Article

Isolation of a yeast mutant deficient in pyruvate carboxylase activity.

Biochemistry international - 1 Mar 1991

Walker M E, Wallace J C

Abstract excerpt

To improve our understanding of the catalytic mechanism and regulatory properties of pyruvate carboxylase (EC 6.4.1.1), an important biotin-dependent enzyme, we have sought to isolate mutants in Saccharomyces cerevisiae which are defective in pyruvate carboxylase activity. One mutant was isolated...

Topics

Base Sequence
Blotting, Western
Cloning, Molecular
Crosses, Genetic
Gene Expression
Genes
Genetic Complementation Test
Molecular Sequence Data
Mutation
Pyruvate Carboxylase
Regulatory Sequences, Nucleic Acid
Saccharomyces cerevisiae

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