Article
Physiological characterisation of a pyruvate-carboxylase-negative Saccharomyces cerevisiae mutant in batch and chemostat cultures.
Antonie van Leeuwenhoek - 1 Nov 1998
de Jong-Gubbels P, Bauer J, Niederberger P, Stückrath I, Kötter P, van Dijken J P, Pronk J T
Abstract excerpt
A prototrophic pyruvate-carboxylase-negative (Pyc-) mutant was constructed by deleting the PYC1 and PYC2 genes in a CEN.PK strain of Saccharomyces cerevisiae. Its maximum specific growth rate on ethanol was identical to that of the isogenic wild type but it was unable to grow in batch cultures in glucose-ammonia media. Consistent with earlier reports, growth on glucose could be restored by supplying aspartate as...
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