Article
Pyruvate carboxylase is an essential protein in the assembly of yeast peroxisomal oligomeric alcohol oxidase.
Molecular biology of the cell - 1 Feb 2003
Ozimek Paulina, van Dijk Ralf, Latchev Kantcho, Gancedo Carlos, Wang Dong Yuan, van der Klei Ida J, Veenhuis Marten
Abstract excerpt
Hansenula polymorpha ass3 mutants are characterized by the accumulation of inactive alcohol oxidase (AO) monomers in the cytosol, whereas other peroxisomal matrix proteins are normally activated and sorted to peroxisomes. These mutants also have a glutamate or aspartate requirement on minimal media. Cloning of the corresponding gene resulted in the isolation of the H. polymorpha PYC gene that encodes pyruvate...
Topics
- Aspartic Acid
- Blotting, Western
- Cytosol
- Glutamic Acid
- Histidine
- Immunohistochemistry
- Microscopy, Electron
- Mutation
- Peroxisomes
- Pichia
- Plasmids