Article
Biochemical and genetic characterization of a yeast TFIID mutant that alters transcription in vivo and DNA binding in vitro.
Molecular and cellular biology - 1 May 1992
Arndt K M, Ricupero S L, Eisenmann D M, Winston F
Abstract excerpt
A mutation in the gene that encodes Saccharomyces cerevisiae TFIID (SPT15), which was isolated in a selection for mutations that alter transcription in vivo, changes a single amino acid in a highly conserved region of the second direct repeat in TFIID. Among eight independent spt15 mutations, seven cause this same amino acid change, Leu-205 to Phe. The mutant TFIID protein (L205F) binds with greater affinity than...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Escherichia coli
- Genes, Fungal
- Genotype
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Phenotype
- Plasmids