Article
Analysis of structure-function relationships of yeast TATA box binding factor TFIID.
Cell - 29 Jun 1990
Horikoshi M, Yamamoto T, Ohkuma Y, Weil P A, Roeder R G
Abstract excerpt
A systematic series of N-terminal, C-terminal, and internal deletion mutants of S. cerevisiae TFIID were expressed in vitro and tested for TATA box binding and basal level transcription activities using, respectively, DNA mobility shift and in vitro transcription assays. The domains responsible for these activities were colocalized to a surprisingly large region containing C-terminal residues 63-240. This region...
Topics
- Base Sequence
- Chromosome Deletion
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Promoter Regions, Genetic
- Protein Binding
- Protein Biosynthesis
- Restriction Mapping
- Saccharomyces cerevisiae