Article
Tissue-specific time courses of spontaneous mutation frequency and deviations in mutation pattern are observed in middle to late adulthood in Big Blue mice.
Environmental and molecular mutagenesis - 1 Jun 2005
Hill Kathleen A, Halangoda Asanga, Heinmoeller Petra W, Gonzalez Kelly, Chitaphan Chaniga, Longmate Jeffrey, Scaringe William A, Wang Ji-Cheng, Sommer Steve S
Abstract excerpt
To better define the time course of spontaneous mutation frequency in middle to late adulthood of the mouse, measurements were made at 10, 14, 17, 23, 25, and 30 months of age in samples of adipose tissue, liver, cerebellum (90% neurons), and the male germline (95% germ cells). A total of 46 million plaque-forming units (pfus) were screened at the six time points and 1,450 circular blue plaques were harvested and...
Topics
- Adipose Tissue
- Age Factors
- Aging
- Animals
- DNA Mutational Analysis
- Germ Cells
- Hepatocytes
- Male
- Mice
- Mice, Mutant Strains
- Mutation
- Neurons
- Organ Specificity