Article
Crystallization of bacteriorhodopsin from bicelle formulations at room temperature.
Protein science : a publication of the Protein Society - 1 Mar 2005
Faham Salem, Boulting Gabriella L, Massey Elizabeth A, Yohannan Sarah, Yang Dawn, Bowie James U
Abstract excerpt
We showed previously that high-quality crystals of bacteriorhodopsin (bR) from Halobacterium salinarum can be obtained from bicelle-forming DMPC/CHAPSO mixtures at 37 degrees C. As many membrane proteins are not sufficiently stable for crystallization at this high temperature, we tested whether the bicelle method could be applied at a lower temperature. Here we show that bR can be crystallized at room temperature...
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