Article
Use of experimental crystallographic phases to examine the hydration of polar and nonpolar cavities in T4 lysozyme.
Proceedings of the National Academy of Sciences of the United States of America - 23 Sept 2008
Liu Lijun, Quillin Michael L, Matthews Brian W
Abstract excerpt
There is conflicting evidence as to whether cavities in proteins that are nonpolar and large enough to accommodate solvent are empty or are occupied by disordered water molecules. Here, we use multiple-wavelength x-ray data collected from crystals of the selenomethionine-substituted L99A/M102L mutant of T4 lysozyme to obtain a high-resolution electron density map free of bias that is unavoidably associated with...
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