Article
X-ray diffraction of a cysteine-containing bacteriorhodopsin mutant and its mercury derivative. Localization of an amino acid residue in the loop of an integral membrane protein.
Biochemistry - 30 Nov 1993
Krebs M P, Behrens W, Mollaaghababa R, Khorana H G, Heyn M P
Abstract excerpt
We have used heavy-atom labeling and X-ray diffraction to localize a single amino acid in the integral membrane protein bacteriorhodopsin (bR). To provide a labeling site, we used the bR mutant, A103C, which contains a unique cysteine residue in the short loop between transmembrane alpha-helices...
Topics
- Amino Acid Sequence
- Bacteriorhodopsins
- Chloromercuribenzoates
- Cysteine
- Fourier Analysis
- Halobacterium salinarum
- Membrane Proteins
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Recombinant Proteins
- X-Ray Diffraction