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Article

Crystallization and preliminary X-ray crystallographic analysis of a blue-light-absorbing proteorhodopsin.

Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Mar 2012

Wang Ning, Wang Meitian, Gao Yanyan, Ran Tingting, Lan Yanli, Wang Jian, Xu Langlai, Wang Weiwu

Abstract excerpt

Proteorhodopsins (PRs), seven-transmembrane chromoproteins with retinal as a chromophore, are light-driven proton pumps. To elucidate the light-driven proton-pumping mechanism of PRs, a pET28a vector containing the blue-light-absorbing proteorhodopsin (BPR) gene was constructed and the protein was overexpressed in Escherichia coli. The protein was purified by immobilized metal-ion affinity chromatography (IMAC)....

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Identifiers

PMID: 22442222
DOI: 10.1107/S1744309111043612
PMCID: PMC3310530

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