Article
Terminal restriction fragment length polymorphism monitoring of genes amplified directly from bacterial communities in soils and sediments.
Molecular biotechnology - 1 Nov 2000
Bruce K D, Hughes M R
Abstract excerpt
Terminal Restriction Fragment Length Polymorphism (T-RFLP) or Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (FluRFLP) have made a significant impact on the way in which PCR products amplified from mixed community DNA extracts have been assessed. Technically, these approaches are essentially the same. PCR products are generated that contain at one 5' end label, typically a...
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