Article
Denaturing gradient gel electrophoresis (DGGE).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Strathdee Fiona, Free Andrew
Abstract excerpt
Denaturing Gradient Gel Electrophoresis (DGGE) is a technique used to separate short- to medium-length DNA fragments based on their melting characteristics. It has been used frequently for identifying single-nucleotide polymorphisms without the need for DNA sequencing and as a molecular fingerprinting method for complex ecosystem communities, in particular in conjunction with amplification of microbial 16S rRNA...
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