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Article

Residual polymerase activity-induced bias in terminal restriction fragment length polymorphism analysis.

Environmental microbiology - 1 Feb 2007

Hartmann Martin, Enkerli Jürg, Widmer Franco

Abstract excerpt

Residual activity of polymerase chain reaction DNA polymerases in restriction analyses strongly affected genetic profiling based on terminal restriction fragment length polymorphisms. Artificial fragment sizes produced as a result of 5'-overhang restriction site fill-in and addition of a terminal A may bias genetic profiling and genotyping of microbial communities. Efficient removal of polymerases retained...

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Identifiers

PMID: 17222153
DOI: 10.1111/j.1462-2920.2006.01169.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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