Article
Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA.
Applied and environmental microbiology - 1 Nov 1997
Liu W T, Marsh T L, Cheng H, Forney L J
Abstract excerpt
A quantitative molecular technique was developed for rapid analysis of microbial community diversity in various environments. The technique employed PCR in which one of the two primers used was fluorescently labeled at the 5' end and was used to amplify a selected region of bacterial genes encoding 16S rRNA from total community DNA. The PCR product was digested with restriction enzymes, and the fluorescently...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.