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Article

Evaluation of terminal-restriction fragment length polymorphism analysis in contrasting marine environments.

FEMS microbiology ecology - 1 Jul 2008

Zhang Rui, Thiyagarajan Vengatesen, Qian Pei-Yuan

Abstract excerpt

Terminal-restriction fragment length polymorphism (T-RFLP) analysis is widely used in microbial ecology studies. In the present study, T-RFLP analysis of PCR products digested by five restriction enzymes (AluI, HaeIII, MspI, Sau3AI and TaqI) was applied for 20 samples from three contrasting coastal environments to assess the biases associated with the choice of enzyme digestion and T-RF analysis. The five enzyme...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 18503550
DOI: 10.1111/j.1574-6941.2008.00493.x

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