Article
Quantifying levels of p53 mutation in mouse skin tumors.
Environmental and molecular mutagenesis - 1 Jun 2005
Verkler Tracie L, Couch Letha H, Howard Paul C, Parsons Barbara L
Abstract excerpt
Allele-specific competitive blocker PCR (ACB-PCR) amplification and quantification was developed for mouse p53 codon 270 CGT-->TGT base substitution and codon 244/245 AAC/CGC-->AAT/TGC tandem mutation. PCR products corresponding to p53 mutant and wild-type DNA sequences were generated. These DNAs were mixed in known proportions to construct samples with defined mutant fractions and the allele-specific detection...
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