Article
A genetic screen for top3 suppressors in Saccharomyces cerevisiae identifies SHU1, SHU2, PSY3 and CSM2: four genes involved in error-free DNA repair.
Genetics - 1 Mar 2005
Shor Erika, Weinstein Justin, Rothstein Rodney
Abstract excerpt
Helicases of the RecQ family and topoisomerase III are evolutionarily conserved proteins important for maintenance of genome stability. In Saccharomyces cerevisiae, loss of the TOP3 gene, encoding topoisomerase III, results in a phenotype of slow growth, DNA damage sensitivity, meiotic defects, and hyperrecombination. The sole RecQ helicase in budding yeast, Sgs1, interacts with Top3 both physically and...
Topics
- Base Sequence
- Cloning, Molecular
- DNA Damage
- DNA Primers
- DNA Repair
- DNA Replication
- DNA Topoisomerases, Type I
- DNA, Fungal
- Genes, Fungal
- Genetic Complementation Test
- Genotype