Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mapping the DNA topoisomerase III binding domain of the Sgs1 DNA helicase.

The Journal of biological chemistry - 23 Mar 2001

Fricke W M, Kaliraman V, Brill S J

Abstract excerpt

Several members of the RecQ family of DNA helicases are known to interact with DNA topoisomerase III (Top3). Here we show that the Saccharomyces cerevisiae Sgs1 and Top3 proteins physically interact in cell extracts and bind directly in vitro. Sgs1 and Top3 proteins coimmunoprecipitate from cell extracts under stringent conditions, indicating that Sgs1 and Top3 are present in a stable complex. The domain of Sgs1...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chromatography, Gel
Share to DNA Helicases
Share to DNA Topoisomerases, Type I

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Enzyme-Linked Immunosorbent Assay

Share to Genetic Complementation Test

Share to Precipitin Tests

Share to Protein Processing, Post-Translational

Share to RecQ Helicases

Source

Open in PubMed Open full text

Identifiers

PMID: 11124263
DOI: 10.1074/jbc.M009719200
PMCID: PMC2818744

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mapping the DNA topoisomerase III binding domain of the Sgs1 DNA helicase.PMID 11124263Read ↗

Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.Semantic proximity Read ↗

Bipartite structure of the SGS1 DNA helicase in Saccharomyces cerevisiae.Semantic proximity Read ↗

The genetic consequences of ablating helicase activity and the Top3 interaction domain of Sgs1.Semantic proximity Read ↗

An essential DNA strand-exchange activity is conserved in the divergent N-termini of BLM orthologs.Semantic proximity Read ↗

A genetic screen for top3 suppressors in Saccharomyces cerevisiae identifies SHU1, SHU2, PSY3 and CSM2: four genes involved in error-free DNA repair.Semantic proximity Read ↗

Sgs1 Binding to Rad51 Stimulates Homology-Directed DNA Repair in Saccharomyces cerevisiae.Semantic proximity Read ↗

Maintenance of Yeast Genome Integrity by RecQ Family DNA Helicases.Semantic proximity Read ↗

Sgs1 regulates gene conversion tract lengths and crossovers independently of its helicase activity.Semantic proximity Read ↗

SGS1, the Saccharomyces cerevisiae homologue of BLM and WRN, suppresses genome instability and homeologous recombination.Semantic proximity Read ↗

Mutations in homologous recombination genes rescue top3 slow growth in Saccharomyces cerevisiae.Semantic proximity Read ↗

Inactivation of homologous recombination suppresses defects in topoisomerase III-deficient mutants.Semantic proximity Read ↗

Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.