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Article

Golgi-to-late endosome trafficking of the yeast pheromone processing enzyme Ste13p is regulated by a phosphorylation site in its cytosolic domain.

Molecular biology of the cell - 1 Mar 2005

Johnston Holly D, Foote Christopher, Santeford Andrea, Nothwehr Steven F

Abstract excerpt

This study addressed whether phosphorylation regulates trafficking of yeast membrane proteins that cycle between the trans-Golgi network (TGN) and endosomal system. The TGN membrane proteins A-ALP, a model protein containing the Ste13p cytosolic domain fused to alkaline phosphatase (ALP), and Kex2p were found to be phosphorylated in vivo. Mutation of the S13 residue on the cytosolic domain of A-ALP to Ala was...

Topics

Alkaline Phosphatase
Amino Acid Sequence
Binding Sites
Biological Transport
Blotting, Western
Cell Membrane
Cytosol
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
Endoplasmic Reticulum
Endosomes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Golgi Apparatus

Immunoprecipitation

Mass Spectrometry

Microscopy, Fluorescence

Models, Biological

Molecular Sequence Data

Mutation

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Source

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Identifiers

PMID: 15647379
DOI: 10.1091/mbc.e04-07-0642
PMCID: PMC551507

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-15647379-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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