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Article

Peroxynitrite-mediated oxidation of the C85S/C152E mutant of dihydrofolate reductase from Escherichia coli: functional and structural effects.

Archives of biochemistry and biophysics - 15 Feb 2005

Pucciarelli Stefania, Spina Michele, Montecchia Francesca, Lupidi Giulio, Eleuteri Anna Maria, Fioretti Evandro, Angeletti Mauro

Abstract excerpt

Peroxynitrite is a potent reactive oxygen species that is believed to mediate deleterious protein modifications in a wide variety of neurodegenerative disorders. In this study, we have analysed the effects of oxidative damage induced by peroxynitrite on a cysteine-free mutant of dihydrofolate reductase (SE-DHFR), from a functional and a structural point of view. The peroxynitrite-mediated oxidation results in the...

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Source

Identifiers

PMID: 15639221
DOI: 10.1016/j.abb.2004.11.008

Genes

DHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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