Article
A novel endoplasmic reticulum membrane protein Rcr1 regulates chitin deposition in the cell wall of Saccharomyces cerevisiae.
The Journal of biological chemistry - 4 Mar 2005
Imai Keita, Noda Yoichi, Adachi Hiroyuki, Yoda Koji
Abstract excerpt
Congo red binds to the cell wall and inhibits the growth of yeast. In a screening for multicopy suppressor genes of Congo red hypersensitivity of erd1Delta mutant, we found that a previously uncharacterized gene, YBR005w, makes most of the Saccharomyces cerevisiae strains resistant to Congo red. This gene was named RCR1 (resistance to Congo red 1). An rcr1Delta null mutant showed an increased sensitivity to Congo...
Topics
- Amino Acid Sequence
- Benzenesulfonates
- Blotting, Western
- Cell Wall
- Chitin
- Coloring Agents
- Congo Red
- Electrophoresis, Polyacrylamide Gel
- Endoplasmic Reticulum
- Escherichia coli
- Fluorescent Antibody Technique, Indirect
- Glucans
- Glycosylation