Article
Sed1p and Srl1p are required to compensate for cell wall instability in Saccharomyces cerevisiae mutants defective in multiple GPI-anchored mannoproteins.
Molecular microbiology - 1 Jun 2004
Hagen Ilja, Ecker Margit, Lagorce Arnaud, Francois Jean M, Sestak Sergej, Rachel Reinhard, Grossmann Guido, Hauser Nicole C, Hoheisel Jörg D, Tanner Widmar, Strahl Sabine
Abstract excerpt
The covalently linked cell wall protein Ccw12p of Saccharomyces cerevisiae is a GPI-anchored protein (V. Mrsa et al., 1999, J Bacteriol 181: 3076-3086). Although only 121 amino acids long, the haemagglutinin-tagged protein released by laminarinase from the cell wall possesses an apparent molecular mass of > 300 kDa. A membrane-bound form with an apparent molecular mass of 58 kDa is highly O- and N-glycosylated...
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