Article
CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae.
The Journal of cell biology - 1 Jul 1991
Valdivieso M H, Mol P C, Shaw J A, Cabib E, Durán A
Abstract excerpt
The CAL1 gene was cloned by complementation of the defect in Calcofluor-resistant calR1 mutants of Saccharomyces cerevisiae. Transformation of the mutants with a plasmid carrying the appropriate insert restored Calcofluor sensitivity, wild-type chitin levels and normal spore maturation. Southern blots using the DNA fragment as a probe showed hybridization to a single locus. Allelic tests indicated that the cloned...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Chitin Synthase
- Cloning, Molecular
- Fungal Proteins
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Plasmids
- Saccharomyces cerevisiae