Article
A simple method for the detection and genotyping of high-risk human papillomavirus using seminested polymerase chain reaction and reverse hybridization.
Gynecologic oncology - 1 Jan 2005
Lin Hao, Moh Jau-Sung, Ou Yu-Che, Shen Shu-Yun, Tsai Yi-Min, ChangChien Chan-Chao, Liu Jacqueline M, Ma Yen-Ying
Abstract excerpt
BACKGROUND: To develop a simple and cost-effective method for the detection and genotyping of high-risk human papillomaviruses (HPV) using seminested polymerase chain reaction (PCR) and reverse hybridization. METHODS: Cervical swabs for HPV testing were collected from 127 women with normal cervical cytology and 57 patients with cervical lesions of various degrees. After DNA isolation, PCR amplification was first...
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