Article
A simple method for the detection and genotyping of high-risk human papillomavirus using seminested polymerase chain reaction and reverse hybridization.
Gynecologic oncology - 1 Jan 2005
Lin Hao, Moh Jau-Sung, Ou Yu-Che, Shen Shu-Yun, Tsai Yi-Min, ChangChien Chan-Chao, Liu Jacqueline M, Ma Yen-Ying
Abstract excerpt
BACKGROUND: To develop a simple and cost-effective method for the detection and genotyping of high-risk human papillomaviruses (HPV) using seminested polymerase chain reaction (PCR) and reverse hybridization. METHODS: Cervical swabs for HPV testing were collected from 127 women with normal cervical cytology and 57 patients with cervical lesions of various degrees. After DNA isolation, PCR amplification was first...
Topics
- Cell Line, Tumor
- Female
- Genotype
- Humans
- Neoplasm Staging
- Nucleic Acid Hybridization
- Oligonucleotide Array Sequence Analysis
- Papillomaviridae
- Polymerase Chain Reaction
- Sensitivity and Specificity