Article
Easy and fast detection and genotyping of high-risk human papillomavirus by dedicated DNA microarrays.
Journal of virological methods - 1 Nov 2006
Albrecht Valérie, Chevallier Anne, Magnone Virginie, Barbry Pascal, Vandenbos Fanny, Bongain André, Lefebvre Jean-Claude, Giordanengo Valérie
Abstract excerpt
Persistent cervical high-risk human papillomavirus (HPV) infection is correlated with an increased risk of developing a high-grade cervical intraepithelial lesion. A two-step method was developed for detection and genotyping of high-risk HPV. DNA was firstly amplified by asymmetrical PCR in the presence of Cy3-labelled primers and dUTP. Labelled DNA was then genotyped using DNA microarray hybridization. The...
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