Article
[The development and clinical application of papillomavirus genotyping by DNA chip].
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Jan 2006
Yang Guang, Liang Cai-hong, Cui Jin-huan, Chen Shu
Abstract excerpt
OBJECTIVE: To develop a new platform for genotyping human papillomavirus (HPV) and to investigate its effect in clinical application. METHODS: A pair of common primers of 18 HPV subtypes for PCR, was designed in HPV conservative L1 region. Genotyping probes for detecting 15 high-risk HPV subtypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68, together with 3 low-risk HPV 6, 11 and 42 were selected...
Topics
- DNA Probes, HPV
- DNA, Viral
- Female
- Genotype
- Humans
- Molecular Sequence Data
- Oligonucleotide Array Sequence Analysis
- Papillomaviridae
- Papillomavirus Infections
- Polymerase Chain Reaction
- Sensitivity and Specificity