Article
Establishment of an efficient multiplex real-time PCR assay for human papillomavirus genotyping in cervical cytology specimens: comparison with hybrid capture II.
Cytopathology : official journal of the British Society for Clinical Cytology - 1 Aug 2011
Lee J-H, Lee N-W, Hong S-W, Nam Y-S, Choi J-W, Kim Y-S
Abstract excerpt
OBJECTIVE: To establish an efficient multiplex real-time PCR assay for 15 human papillomavirus (HPV) genotypes, we designed multiplexing parameters and compared our PCR system with the hybrid capture (HC) II test using cervical cytology samples. METHODS: For preventing cross-reactive amplificatio...
Topics
- Female
- Genotype
- Humans
- Papillomaviridae
- Papillomavirus Infections
- Polymerase Chain Reaction
- Reproducibility of Results
- Uterine Cervical Neoplasms
