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Article

Establishment of an efficient multiplex real-time PCR assay for human papillomavirus genotyping in cervical cytology specimens: comparison with hybrid capture II.

Cytopathology : official journal of the British Society for Clinical Cytology - 1 Aug 2011

Lee J-H, Lee N-W, Hong S-W, Nam Y-S, Choi J-W, Kim Y-S

Abstract excerpt

OBJECTIVE: To establish an efficient multiplex real-time PCR assay for 15 human papillomavirus (HPV) genotypes, we designed multiplexing parameters and compared our PCR system with the hybrid capture (HC) II test using cervical cytology samples. METHODS: For preventing cross-reactive amplifications, variable HPV genes (E1, E2, E6, E7 and L1) were targeted. The melting temperatures of all primers and probes, and...

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PMID: 20636403
DOI: 10.1111/j.1365-2303.2010.00787.x

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