Article
Multilocus sequence typing analysis of human and animal Clostridium difficile isolates of various toxigenic types.
Journal of clinical microbiology - 1 Jun 2004
Lemee Ludovic, Dhalluin Anne, Pestel-Caron Martine, Lemeland Jean-François, Pons Jean-Louis
Abstract excerpt
A multilocus sequence typing (MLST) scheme was developed to study the genetic relationships and population structure of 72 Clostridium difficile isolates from various hosts, geographic sources, PCR ribotypes, and toxigenic types (determined by PCR targeting tcdA and tcdB genes). MLST was performed by DNA sequence analysis of seven housekeeping genes (aroE, ddl, dutA, tpi, recA, gmk, and sodA). The number of...
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