Article
Rapid detection of rifampin resistance in Mycobacterium tuberculosis isolates from India and Mexico by a molecular beacon assay.
Journal of clinical microbiology - 1 Dec 2004
Varma-Basil Mandira, El-Hajj Hiyam, Colangeli Roberto, Hazbón Manzour Hernando, Kumar Sujeet, Bose Mridula, Bobadilla-del-Valle Miriam, García Lourdes García, Hernández Araceli, Kramer Fred Russell, Osornio Jose Sifuentes, Ponce-de-León Alfredo, Alland David
Abstract excerpt
We assessed the performance of a rapid, single-well, real-time PCR assay for the detection of rifampin-resistant Mycobacterium tuberculosis by using clinical isolates from north India and Mexico, regions with a high incidence of tuberculosis. The assay uses five differently colored molecular beacons to determine if a short region of the M. tuberculosis rpoB gene contains mutations that predict rifampin resistance...
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